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KMID : 0613820170270111256
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Journal of Life Science
2017 Volume.27 No. 11 p.1256 ~ p.1261
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β-glucan Stimulates Release of TNF-α in Human Monocytic THP-1 Cells
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Keum Bo-Ram
Hyeon Jin-Yi
Choe So-Hui
Jin Ji-Young
Jeong Ji-Woo
Lim Jong-Min
Park Dong-Chan
Cho Kwang-Keun
Choi Eun-Young
Choi In-Soon
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Abstract
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β-glucan is a constituent of the cell wall of fungi, yeast and plants. It plays an important role in the immune system such as activation of immunocyte, release of pro-inflammatory and anti-cancer effect. The immune system maintains a healthy immune homeostasis. However, when pathogenic substances enter the body, immune homeostasis can break down and disease can be triggered. Therefore, we studied a substance that regulates immune homeostasis. The purpose of the study we demonstrated whether the β-glucan can be applied to the immune-modulation effects in human monocytic THP-1 cells. β-glucan was incubated in THP-1 cells at various concentrations. The TNF-α mRNA expression and protein levels were analyzed by ELISA and Real-time PCR. Additionally, the expression of MAPKs (p38 and JNK), IκB-α and NF-κB p50 were analyzed by western blot. β-glucan enhanced the production of TNF-α mRNA expression and protein levels in human monocytic THP-1 cells. In addition, activation of MAPKs (p38 and JNK) and NF-κB p50 induced by β-glucan were increased. The study suggests that β-glucan contributes to immune-stimulation effect by production TNF-α in human monocytic THP-1 cells, and that MAPKs and NF-κB p50 are involved in the process. Synthetically, we have suggested β-glucan may be improved to immune system effect in human monocytic THP-1 cells.
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KEYWORD
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β-glucan, immunomodulation, NF-κB p50, THP-1, TNF-α
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